LOCUS       pETDuetMmPPTaseCb1CAR      9325 bp    DNA     linear   UNA 04-Mai-2021
DEFINITION  pETDuet1MmPPTase
FEATURES             Location/Qualifiers
     CDS             71..757
                     /cds_type="ORF"
                     /label="MmPPTase WP_094357779"
     misc_feature    964..981
                     /label="HIS Tag"
     CDS             1027..4221
                     /cds_type="ORF"
                     /label="Clostridium bornimense CAR (NADH)"
ORIGIN
        1 GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG
       61 GAGATATACC ATGGGCACCA TCAGCATGCT GGTTAGCAGC GTTCTGCCGG GTACCGTGGT
      121 TGACGATCTG GCGTACGCGG AACTGTATAG CGACCCGCCG GGTCTGCTGC CGCTGCCGGA
      181 GGAAGAGCCG CTGATCGCGC GTAGCGTTGC GAAGCGTCGT AACGAGTTCA TTACCGTTCG
      241 TCACTGCGCG CGTGTGGCGC TGGGTGATCT GGGCGTGCCG CCGGTTCCGA TCCTGAAGGG
      301 TGACAAAGGC CAGCCGTGCT GGCCGGATGG TGTGGTTGGC AGCCTGACCC ATTGCAGCGG
      361 TTACCGTGGC GCGGTGGTTG GTCGTAGCGC GGCGGTGCGT AGCGTTGGTA TTGACGCGGA
      421 ACCGCACGAT GTGCTGCCGA ACGGTGTTCT GGACGCGATC AGCCTGCCGG AAGAGCGTGA
      481 TGAAATTCCG AGCGCGATGC CGGATGGTCT GCACTGGGAT CGTATCCTGT TCTGCGCGAA
      541 AGAGGCGACC TATAAAGTTT GGTTTCCGCT GACCAACCGT TGGCTGGGTT TCGAAGACGC
      601 GCACATTACC TTTGAGGCGG ACGATAGCGG TCGTACCGGC CGTTTTGTGA GCCGTATCCT
      661 GATTGATCCG AGCGCGCTGT GGGGCCCGCC GCTGACCACC CTGCACGGTC GTTGGAGCGT
      721 GGAGCGTGGC CTGGTTCTGA CCGCGATTGT GCTGTGAGGA TCCGAATTCG AGCTCGGCGC
      781 GCCTGCAGGT CGACAAGCTT GCGGCCGCAT AATGCTTAAG TCGAACAGAA AGTAATCGTA
      841 TTGTACACGG CCGCATAATC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT
      901 AACAATTCCC CATCTTAGTA TATTAGTTAA GTATAAGAAG GAGATATACA TATGAGCTAC
      961 TACCATCACC AcCACCAcCA tGATTACGAC ATCCCAACGA CCGAAAACCT GTATTTTCAG
     1021 GGCGCCATGA AATATTACAT TCAAGAAAAT CAGGAGAACT TTTATAATTT ACTTTCAATT
     1081 AATGCCAAAA AACACGATGA ATGCTTCACG TTTGTGCACT ACATTGATGA CAAAAAAGTG
     1141 AAAGTTCAGC TTACTCGTTC GGATCTGTTA AAAAAGTCAA TCGACGTTGC TGCTAAGCTG
     1201 AAGGCTGCGG GGATTAAAAA GGGGGACCGT GTGATTATTC TTAGCACTCA GACCGTTGAT
     1261 AATGTATTAA GCGTTGTGGG GTCAATGTTC GCCGGTGCTA TTTTCACCAT CATCCCTCCA
     1321 CCGATAGATT CTTCCAAGAT GGAGCGTTTT ATTAGCGTTG TCAAATCATG TAACCCGAAA
     1381 TGCTTGCTCT GCGGAAATAT GCTTGAAAAT AAATCTAAAG AAATTCTTAG CACACTTAAA
     1441 AAAAAAGCTG AAGTTGCAGA GATTTTTTCG AACCTGGACG TCATCAATGT GGAGAACTGC
     1501 GAGGAAGCTG TTGATTTTAC CCCTGAGAAG ATTGACCTGG ATGACATCAT TTATATTCAG
     1561 TATAGCTCAG GCTCCACTTC TGCTCCGAAG GGGGTATTAA TTAGTTATGG CAATTTGATT
     1621 TCTGCAGTTA ACACGACCGT CGAACCACTC GGCGCTGAAA GAGTATTTGG GTGGGTACCG
     1681 TTCTTTCATA ACCTGGGTCT GGTTTATTTA ATTTTTACAT CGATTATCAA ACCGGAATTT
     1741 GTTGTTGGAA TCATGTCACC CGCGGACTTC CTGGCAAAAC CTTCCCGTTG GATTGAAAAC
     1801 TTGAGTGAAT TCAAAGCAGA TACTACCTTA GCACCGAATT CAGTCTATGA GAGTTATCCG
     1861 AAACTGGTCC CTGCGAGTAA ATTAAAGGGG ATAGATCTTT CTAACCTGCG ACTCTTCTTA
     1921 AACGGCAGCG AGATAGTTAC TTATAGCTCA ATGAAACAAT TCGCGGAAGA ATATAAAGAT
     1981 TTTGGTGTTT CGATTAATGA TTTCATGGTT GGTTATGGCC TGGCCGAAGT TACCTGTGGG
     2041 GTGACCGGCG TTCGTAATTT TTCAGAAGAT GAAGTACTGG AATTGGACTT CGATCAGTAT
     2101 CAGAACGGGA AACTGGTTCT GGCAAACGAG AATACAGAAA ATAAAATTGA ATTCATCGGG
     2161 AATGGTTATA CGGTAAAGGA CCATAAAATC ATAGCTGTCA ACCCCGATAC TCTGGAGCCG
     2221 TGCAAAGAAG ACGAATTTGG GGAGCTGTGG ATCAAAGGCC CCGCCGTTGC TAAAGGCTAC
     2281 TACAATAACG CCAAAGCTAC CAAAGAGACC TTTCATAACA AACTTAAGGG ATATGAGGAA
     2341 GACTTTCTGC GTACAGGTGA TCTGGGCATA ATCAAAGATG ATTACGTATA TATTACGGGC
     2401 CGCATGAAGG AACTTATTAT TATTAACGGA AACAATATTC TGCCAAACGA CATAGTAGTC
     2461 AAGTTGACGG AAAAAATGCC GGCGCTGAAA TATGCCAATA TTGTCCCGTT TTCAATAGTT
     2521 AAAGAGGAAA AAGAACGTTT GATTATTGCG CTGGAGATTC CGGAGAGTCT TAAAGATGTT
     2581 GTTAAGTTTG ATGATGTGGT CGAGATGATT AACAAAGCTG TCTTAGAATA TTTTGAGGTC
     2641 TCACCGTATG AAATCTGTGT TGTGGCAACG GGTGCACTGC CAAAAAGCGA TAACGGCAAG
     2701 GTTTCAATTG CTAAGACCCG AAAACTGTAT GAAGAAGATA AGCTGAACAT AGTTATATCA
     2761 TCTAGAAATG AGGAATCACA GTACGAGAAA AACATTGAGT ATGATACAGA GACGGAGAAA
     2821 GTCTTGGGGA CGATTATAAA CGAGGAATTT AATCATAAAG CAGATAGAGA CGACAACCTG
     2881 CTTAACATGG GTATGGATAG TCTGGAAGTC ACACAGCTGA CAACCAAGAT TGAAGAAAAA
     2941 TTTAATGTAA ATGTACCGGT GTCATTCATA TTTGAAAACC CCACCATCAA TAAGATCTCG
     3001 AAATATATAG ATCGAACCCT GGCTGGTGAA GATATAGCAG ATCTGGAAAA GGACAAAAGT
     3061 TACTTATACG ATGAAGTGAA ACTGGACGAG AATATTAAAC CAGGCAAATA CGAAGACGAC
     3121 GCGCCCGCGA TGAAAAACGT CTTTATCACG GGTACAACTG GGTTTGTCGG AGCCTATCTG
     3181 ATTCACTCTT TGTTATCGGA GACGGAGGCG CGGCTGTATT GCCATGTGCG CGCAAAAAAT
     3241 GAAGAAGAGG CACTTAAGAG AATTAAATAT AACATGAAGT ACTATAATCT CTGGAAGGAA
     3301 GAATACCGTG AATTTATTAC CCCGGTGGTA GGGTCCTTAG ATGAGCCCCT TCTGGGCATC
     3361 GAGGAAGAGA AGTATAAAAT GCTGACTGAG ACCATTGATA CCGTGTACCA CAATGGAGCC
     3421 CTGCTGAACT TTATTTATCC GTATGAACGC TTAAAAAACA CGAATGTTGG CGGTACTATT
     3481 AAAACCCTGA AATTCGCATG CACAGGAAAA CCTAAATACT ACAACTATGT TAGCAGCTAT
     3541 TCAGTTTTCG ATAACCCGAG TCATTTCGAG AAGGTGGCTA TGGAAGATGA TCCTATCGAC
     3601 TCATGCATTG GTTATTATCT CTCGTATTCA GAAACCAAAT GGGTGTGTGA AAAAATAGTC
     3661 AATCTGGCCC GCAAAGCGGG CCTTAGAGCG GCCATTTATC GCCCAGGGGA AATTACCGGC
     3721 GCAAACGACA CGGGCATCTG GAAATTATCT GATTCCATCA GCCGCACGTT CAAGTCAATC
     3781 CTGCAGACAA AAGTTTATCC AAACATTGAG TTGAAGATCC ACATGACTCA GGTTGATTAT
     3841 ATTGCAGAAG CTATTGTGAA AATTAGCAAA CAGGGAGATG CCTATGGTAA AGCATACAAT
     3901 CTGATGAACT CCGTTTACGT TTCTATAGGC GAATTAGGGC GCATTATTAA TAAATGCGGC
     3961 TATGAAGCTA AACCGCTCAG TTATGAAGAA TGGAAGGAAA ATTTATTCAG CTCCGGGAAC
     4021 GAGCATCCGC TGAAGCTGTT AGAATCTCTG TTCCGAATAA CGAAAAAAAA TGAAAAGGAA
     4081 GAATTTATTA ATCGCTATGG GAAAATGAGC CCCACCCATT TTGATACAAC TAATACTGAT
     4141 AAAGCACTGG AAGGCACTGG TCTGTCCTGT GAACCGATGA ACGAGGCGCT TATAGAAAAA
     4201 TACATCAAAA ACTTCATCTA AGATATCGGC CGGCCACGCG ATCGCTGACG TCGGTACCCT
     4261 CGAGTCTGGT AAAGAAACCG CTGCTGCGAA ATTTGAACGC CAGCACATGG ACTCGTCTAC
     4321 TAGCGCAGCT TAATTAACCT AGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT
     4381 TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATT
     4441 GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC
     4501 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC
     4561 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG
     4621 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA
     4681 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC
     4741 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT
     4801 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA
     4861 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTCTGG CGGCACGATG
     4921 GCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA
     4981 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG
     5041 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG
     5101 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC
     5161 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG
     5221 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG
     5281 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG
     5341 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT
     5401 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC
     5461 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC
     5521 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA
     5581 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC
     5641 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT
     5701 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC
     5761 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA
     5821 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA
     5881 TACTCTTCCT TTTTCAATCA TGATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     5941 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGTC ATGACCAAAA TCCCTTAACG
     6001 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
     6061 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
     6121 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
     6181 AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
     6241 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
     6301 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
     6361 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
     6421 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
     6481 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
     6541 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
     6601 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
     6661 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC
     6721 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG
     6781 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA
     6841 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA
     6901 ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG
     6961 TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC
     7021 TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT
     7081 TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT
     7141 GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA
     7201 GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG
     7261 TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC
     7321 GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT
     7381 GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC
     7441 AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG
     7501 CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG
     7561 AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC
     7621 AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC
     7681 GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCTAGTCATG CCCCGCGCCC
     7741 ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGAGAT CCCGGTGCCT
     7801 AATGAGTGAG CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA
     7861 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA
     7921 TTGGGCGCCA GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC
     7981 ACCGCCTGGC CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA
     8041 AAATCCTGTT TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG
     8101 TATCCCACTA CCGAGATGTC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT
     8161 GCGCCCAGCG CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC
     8221 AGCATTTGCA TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT
     8281 ATCGGCTGAA TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC
     8341 GAGACAGAAC TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA TGCGACCAGA
     8401 TGCTCCACGC CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT GATGGGTGTC
     8461 TGGTCAGAGA CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC CACAGCAATG
     8521 GCATCCTGGT CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG CGCGAGAAGA
     8581 TTGTGCACCG CCGCTTTACA GGCTTCGACG CCGCTTCGTT CTACCATCGA CACCACCACG
     8641 CTGGCACCCA GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA CGGCGCGTGC
     8701 AGGGCCAGAC TGGAGGTGGC AACGCCAATC AGCAACGACT GTTTGCCCGC CAGTTGTTGT
     8761 GCCACGCGGT TGGGAATGTA ATTCAGCTCC GCCATCGCCG CTTCCACTTT TTCCCGCGTT
     8821 TTCGCAGAAA CGTGGCTGGC CTGGTTCACC ACGCGGGAAA CGGTCTGATA AGAGACACCG
     8881 GCATACTCTG CGACATCGTA TAACGTTACT GGTTTCACAT TCACCACCCT GAATTGACTC
     8941 TCTTCCGGGC GCTATCATGC CATACCGCGA AAGGTTTTGC GCCATTCGAT GGTGTCCGGG
     9001 ATCTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG
     9061 GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC
     9121 CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG
     9181 GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT
     9241 GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCG ATCTCGATCC
     9301 CGCGAAATTA ATACGACTCA CTATA
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