LOCUS       Exported File           9576 bp ds-DNA    circular SYN 25-OKT-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pETDuet_MmPPTase_MmCAR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dienstag, 25. Aug 2020 from SnapGene 1.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9576
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    3..27
                     /note="lac operator"
                     /note="color: #31849b"
     misc_feature    71..754
                     /note="MmPPTase_IDT"
                     /note="color: #33cccc"
     promoter        824..842
                     /note="T7 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    843..867
                     /note="lac operator"
                     /note="color: #31849b"
     misc_feature    892..909
                     /note="ribosome binding site"
                     /note="color: #339966"
     CDS             922..939
                     /codon_start=1
                     /note="6xHis"
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     misc_feature    961..981
                     /note="TEV cleavage site"
                     /note="color: #3366ff"
     misc_feature    985..4509
                     /note="MmCAR"
                     /note="color: #993366"
     CDS             4522..4566
                     /codon_start=1
                     /note="S-Tag"
                     /note="color: #cc99b2"
                     /translation="KETAAAKFERQHMDS"
     terminator      4618..4665
                     /note="T7 terminator"
                     /note="color: #ffffff"
     rep_origin      4702..5157
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="color: #ffff00"
     CDS             complement(5272..6132)
                     /codon_start=1
                     /note="AmpR"
                     /note="This feature has 2 segments:
                        1: 5272 .. 6063 / #ccffcc
                        2: 6064 .. 6132 / #ccffcc / signal sequence
                     Cleavage site after base 6063"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6133..6225)
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      6306..6894
                     /direction=RIGHT
                     /note="ori"
                     /note="color: #ffff00"
     CDS             complement(7324..7515)
                     /codon_start=1
                     /note="rop"
                     /note="color: #993366"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8087..9169)
                     /codon_start=1
                     /note="lacI"
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(9170..9247)
                     /note="lacI promoter"
                     /note="color: #ffffff; direction: LEFT"
     promoter        9560..2
                     /note="T7 promoter"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgacgatca gcatgcttgt aagttccgtt ttacccggca ctgtcgtgga
      121 cgatttagct tatgcggaac tgtactccga cccacccggc cttcttccac ttccggagga
      181 ggaaccgctt attgcccgta gtgtcgcaaa gcgccgtaat gagttcatca ctgtccgtca
      241 ttgtgcgcgt gtggccttgg gggatttagg tgtgcctccc gttcctattt taaaggggga
      301 taagggacag ccttgctggc ctgatggggt ggtaggctcg ttgacacatt gcagtggcta
      361 ccgtggcgcg gttgttgggc gtagcgccgc tgttcgctct gtcggcattg acgcagaacc
      421 tcatgacgtt ttacctaacg gtgtgttgga tgctatcagt ttacctgagg aacgcgacga
      481 aattccgtct gcgatgcccg atggattgca ctgggaccgt atcctgtttt gtgcaaagga
      541 agcaacttac aaagtgtggt tccctttaac gaatcgttgg cttggttttg aagatgctca
      601 tatcactttc gaggcggacg attctggtcg caccggacgc ttcgtttcac gtatcctgat
      661 tgatccctcg gcgttgtggg gcccacctct taccacgctt catggtcgct ggagtgtaga
      721 acgcgggctg gtgctgaccg caatcgttct gtaagcttgc ggccgcataa tgcttaagtc
      781 gaacagaaag taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata
      841 ggggaattgt gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga
      901 gatatacata tgtcgtacta ccatcaccat caccatcacg attacgacat cccaacgacc
      961 gaaaacctgt attttcaggg cgccatgtca ccgattaccc gcgaagaacg cctggaacgc
     1021 cgcattcaag acctgtacgc caacgacccg caattcgccg ccgcaaaacc ggcgaccgcc
     1081 attacggcag caatcgaacg tccgggtctg ccgctgccgc aaattatcga aaccgtgatg
     1141 acgggttatg cagatcgtcc ggcactggct cagcgctccg tcgaatttgt gaccgatgcc
     1201 ggcacgggtc ataccacgct gcgtctgctg ccgcacttcg aaaccattag ttacggtgaa
     1261 ctgtgggacc gcatctcagc gctggccgat gtgctgtcga ccgaacagac ggtgaaaccg
     1321 ggcgatcgtg tttgcctgct gggttttaac tcggttgact atgcaaccat tgatatgacg
     1381 ctggctcgtc tgggtgcagt cgctgtgccg ctgcaaacct cagcagctat tacgcagctg
     1441 caaccgatcg ttgcggaaac ccagccgacg atgatcgcag ccagtgtcga tgcactggca
     1501 gacgcaaccg aactggcact gtccggccaa accgctacgc gcgttctggt cttcgaccat
     1561 caccgtcagg tggatgcaca tcgcgcagct gttgaaagcg ctcgtgaacg cctggcaggc
     1621 tctgctgtgg ttgaaaccct ggctgaagcg attgcccgtg gtgatgtgcc gcgcggtgca
     1681 agcgctggtt ctgcaccggg taccgatgtt agtgatgact ccctggcact gctgatctat
     1741 acctcaggtt cgacgggtgc accgaaaggt gcaatgtacc cgcgtcgcaa cgtggcaacc
     1801 ttttggcgta aacgcacgtg gttcgaaggc ggttatgaac cgagcattac cctgaacttt
     1861 atgccgatgt ctcatgttat gggccgtcag atcctgtatg gtaccctgtg taatggcggt
     1921 acggcatact ttgttgctaa atcagacctg tcgaccctgt tcgaagatct ggcgctggtg
     1981 cgtccgaccg aactgacgtt cgttccgcgc gtctgggata tggtttttga cgaattccaa
     2041 tctgaagttg atcgtcgcct ggtcgatggt gcagaccgtg tcgcactgga agcacaggtg
     2101 aaagccgaaa ttcgtaatga tgtgctgggc ggtcgctata ccagcgcact gacgggtagt
     2161 gcaccgatct ccgacgaaat gaaagcctgg gtcgaagaac tgctggatat gcatctggtg
     2221 gaaggctacg gttctaccga agcaggcatg attctgatcg acggtgcaat tcgtcgcccg
     2281 gctgtgctgg attataaact ggtggatgtt ccggacctgg gctactttct gaccgaccgt
     2341 ccgcacccgc gcggtgaact gctggttaaa acggatagcc tgttcccggg ttattaccag
     2401 cgtgcggaag tgaccgccga cgtttttgat gcagacggct tctatcgcac gggtgatatc
     2461 atggcggaag ttggcccgga acaatttgtc tacctggatc gtcgcaacaa tgtgctgaaa
     2521 ctgagtcagg gtgaatttgt caccgtgtcc aaactggaag cggtcttcgg cgattcaccg
     2581 ctggtgcgtc aaatttatat ctacggtaac tcggcgcgtg cgtatctgct ggccgttatt
     2641 gtcccgaccc aggaagcact ggatgctgtg ccggttgaag aactgaaagc acgtctgggc
     2701 gatagcctgc aggaagttgc gaaagcggcc ggtctgcaat cttacgaaat cccgcgcgat
     2761 tttattatcg aaaccacgcc gtggaccctg gaaaatggcc tgctgacggg tattcgtaaa
     2821 ctggcgcgcc cgcagctgaa aaaacattat ggcgaactgc tggaacagat ctacaccgac
     2881 ctggcacacg gccaagctga tgaactgcgt tcactgcgcc agtcgggtgc agatgctccg
     2941 gtcctggtga ccgtttgccg tgcagctgca gcactgctgg gcggtagcgc atctgatgtg
     3001 cagccggacg cccattttac cgatctgggc ggtgacagtc tgtccgcgct gagctttacg
     3061 aacctgctgc acgaaatttt cgatatcgaa gtcccggtgg gcgttattgt ctctccggcc
     3121 aatgacctgc aagcgctggc cgattatgtg gaagcagctc gtaaaccggg tagctctcgc
     3181 ccgacctttg cgagcgttca tggcgcctct aacggtcagg tgaccgaagt tcacgcgggc
     3241 gatctgagcc tggacaaatt cattgatgca gcaaccctgg cagaagctcc gcgtctgccg
     3301 gcagctaata cccaggtccg tacggtgctg ctgaccggtg caacgggttt tctgggccgt
     3361 tatctggctc tggaatggct ggaacgcatg gatctggtgg acggtaaact gatctgtctg
     3421 gttcgtgcga aatcagacac cgaagcgcgt gcccgcctgg ataaaacgtt cgattcgggc
     3481 gacccggaac tgctggccca ttatcgcgca ctggctggtg accacctgga agtgctggcg
     3541 ggtgataaag gcgaagccga cctgggcctg gatcgtcaga cctggcaacg cctggcggat
     3601 acggtcgacc tgattgtgga tccggcggcc ctggttaacc atgtcctgcc gtatagtcag
     3661 ctgtttggcc cgaatgcgct gggtaccgcc gaactgctgc gtctggcact gacgtccaaa
     3721 atcaaaccgt atagctacac ctctacgatt ggcgtggcgg atcagatccc gccgtcagca
     3781 ttcaccgaag atgctgacat tcgtgttatc tcagcgacgc gcgccgtcga tgactcgtat
     3841 gcgaacggct actcaaattc gaaatgggcg ggtgaagtgc tgctgcgtga agcacatgat
     3901 ctgtgcggtc tgccggtggc agtttttcgc tgtgacatga ttctggcgga taccacgtgg
     3961 gccggtcagc tgaacgttcc ggatatgttt acccgcatga ttctgagtct ggcagctacg
     4021 ggtatcgcac cgggttcctt ctatgaactg gcagcagacg gtgcacgtca gcgtgctcac
     4081 tacgatggtc tgccggtgga atttattgcg gaagccatca gtaccctggg cgcccagtcc
     4141 caagatggtt tccatacgta tcacgtcatg aacccgtacg atgacggcat tggtctggac
     4201 gaatttgtgg attggctgaa tgaaagtggc tgcccgattc aacgtatcgc ggattatggt
     4261 gactggctgc agcgtttcga aaccgcactg cgcgctctgc cggatcgtca acgccatagt
     4321 tccctgctgc cgctgctgca caattaccgt cagccggaac gtccggttcg cggcagcatt
     4381 gcaccgaccg atcgttttcg cgcagctgtc caggaagcaa aaattggtcc ggataaagac
     4441 atcccgcatg ttggtgctcc gattattgtc aaatatgtca gtgatctgcg tctgctgggt
     4501 ctgctgtaac tcgagtctgg taaagaaacc gctgctgcga aatttgaacg ccagcacatg
     4561 gactcgtcta ctagcgcagc ttaattaacc taggctgctg ccaccgctga gcaataacta
     4621 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     4681 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4741 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4801 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4861 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4921 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4981 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5041 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5101 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg
     5161 gcggcacgat ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     5221 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     5281 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     5341 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     5401 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     5461 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     5521 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     5581 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     5641 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5701 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     5761 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5821 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5881 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5941 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6001 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     6061 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     6121 ttgaatactc atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc
     6181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa
     6241 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6301 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6361 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6421 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6481 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6541 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6601 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6661 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6721 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6781 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6841 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6901 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6961 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7021 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7081 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca
     7141 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     7201 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     7261 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     7321 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     7381 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     7441 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     7501 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     7561 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     7621 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
     7681 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
     7741 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
     7801 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
     7861 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
     7921 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat
     7981 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
     8041 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
     8101 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8161 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     8221 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     8281 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
     8341 cggtatcgtc gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa
     8401 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
     8461 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     8521 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     8581 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
     8641 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
     8701 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
     8761 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
     8821 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     8881 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     8941 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
     9001 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
     9061 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
     9121 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
     9181 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
     9241 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     9301 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     9361 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     9421 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     9481 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
     9541 gatctcgatc ccgcgaaatt aatacgactc actata
//
