LOCUS       pET28b_AoxDH_BrJ2       6331 bp    DNA     linear       22-JAN-2021
FEATURES             Location/Qualifiers
     misc_feature    5083..5100
                     /note="6xHIS"
     misc_feature    5057..5064
                     /note="RBS"
     misc_feature    5128..6294
                     /note="OxdTv"
BASE COUNT     1583 a   1663 c   1661 g   1544 t
ORIGIN      
          1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
         61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
        121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
        181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
        241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
        301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
        361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
        421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
        481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
        541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
        601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
        661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
        721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
        781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
        841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
        901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
        961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
       1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
       1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
       1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
       1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
       1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
       1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
       1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
       1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
       1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
       1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
       1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
       1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
       1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
       1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
       1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
       1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
       1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
       2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
       2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
       2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
       2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
       2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
       2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
       2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
       2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
       2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
       2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
       2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
       2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
       2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
       2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
       2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
       2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
       3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
       3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
       3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
       3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
       3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
       3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
       3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
       3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
       3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
       3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
       3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
       3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
       3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
       3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
       3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
       3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
       3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
       4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
       4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
       4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
       4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
       4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
       4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
       4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
       4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
       4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
       4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
       4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
       4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
       4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
       4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
       4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
       4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
       4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
       5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       5101 agcagcggcc tggtgccgcg cggcagcCAT ATGAGCCATA GCCAGATTCA GACCGAAAAT
       5161 CGTGAACTGC CGCTGAATCA GCCGGAAAAT TGGCAGGTTA AATTTCCGCG TTATATGGTT
       5221 GAACTGCCGG AACATGTTCA TGATGTTAAC GTTAGCTATA TTGGTATTCA GCCTCGCCAT
       5281 GAAGAAATTC CGGTTCGTGG TAGCCATACC GAACTGAATG CACATGTTAG CAGCGCAGCA
       5341 CAGCGTACCG CAGCAACCAA AATTGAAGAA TGGATTCGTC AGGGTGCCGG TGAACTGGCA
       5401 CCGGTTCTGT GGGAAAAAAC CTATGTTGAA GCAGGTTATG ATGTTCCGGA TACCGAAATC
       5461 TATATTCTGT ATTGGACCGA TAAAGATGTT GCCAAAGCAG CAAATAAAAG CCTGAATCTG
       5521 CAGCAGCTGC ATAAAACCCT GAGCCCGAGC GATCAAGAAA TTGTTGGTGT TTGGCGTGAA
       5581 AGCTTTACCG TTCCGAAACT GCGTTTTGAA ACCGTTTATA GCGGCAATGA TTATCAGCCT
       5641 GGTATTGCAA GCCTGGAAGG TGCACAGCAG GTTAGCCACA AATTTACCGG TTATTGGGGT
       5701 GCAGCACGTG ATCGTTTTCC GGCAAGCAGC CATGATCCGT TTGAACCGGA ATATAAACTG
       5761 AGCACCCCGA ACTTTGATAA AGATAACAAA GGTCGTGTGA TCGAGGGCGA AAATAAAGTT
       5821 AGCGTTCTGC ATATTCGTAG CGGTCAGTAT TGGGAACGTT GTAAAGAAGA TGAACGCGAA
       5881 GCCTATGAAA GCACCCTGGA ACCGGTTCTG CGTGTTGGTA TGACCTATCT GGAAGAAAAT
       5941 GCAGCAGAAA GCGGTGATAT TGGTCTGCGT TTTAGCCGTA ATATGATTAC CAATCGTCTG
       6001 CTGCAGGCAG AAGAAGCACA GCAGCTGGCA AGCCTGACCG AACGTCGTCG TCGTGAAACC
       6061 TGTAGCAGCG GTTTTTTTCG TAGCCTGAAA GATATTGAAG CATGGGCCAG CAAACATAAA
       6121 AGCCACCATA AAATCTTTCA TGGTGCACAT AGCCATAGTC AGCAGTTTGG TAAAGAAGCC
       6181 TTTAAAATGC GTACCTGGCA TGAAGTTAGC GCACTGAAAC CGGGTGAAGT TGCATTTGAA
       6241 TATATCAATT GTTATCCGCG TACCGGTCTG ATCCCGTTTT TTGATGTTCT CGAGcaccac
       6301 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
       6361 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
       6421 tttttgctga aaggaggaac tatatccgga t
//
